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KMID : 0383820080650060504
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Tuberculosis and Respiratory Diseases
2008 Volume.65 No. 6 p.504 ~ p.511
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Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-κB, TGF-β and Fibronectin in WI-26 VA4 Epithelial Cells
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Park Kyeong-Seon
An Chang-Hyeok
Kim Yu-Jin
Kyung Sun-Young
Lee Sang-Pyo
Park Jeong-Woong
Jeong Sung-Hwan
Yoon Jin-Young
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Abstract
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Background: Particulate matter may be toxic to human tissue. Ambient air particulate matter ≤10μm in
aerodynamic size (PM10), which changes under different environmental conditions, is a complex mixture of organic
and inorganic compounds. The Asian dust event caused by meteorological phenomena can also spread unique
particulate matter in affected areas. We evaluated production of ROS, TGF-β, fibronectin, and NFκB by exposing
normal epithelial cells to Asian dust particulate matter.
Methods: Bronchial epithelial cells were exposed to 0, 50, 100μg/ml of a suspension of PM10 for 24 h. ROS were
detected by measurement of DCF release from DCF-DA by FACScan. TGF-β, fibronectin, and NFκB were detected
by western blotting.
Results: PM10 exposure increased the expression of TGF-β, fibronectin, and NFκB. ROS production and TGF-
βlevels were significantly higher with 50 or 100μg/ml PM10. Fibronectin and NFκB production were significantly
higher after 100μg/ml of PM10.
Conclusion: PM10 from Asian dust particles might have fibrotic potential in bronchial epithelial cells via ROS
induction after PM10 exposure.
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KEYWORD
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Particulate matter, Asian dust, Reactive oxygen species, Pulmonary fibrosis
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